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I have an audio file of an owl hooting. The hoots are barely audible. If I look at the spectrogram in Audacity I can see them. They are the blips at 0.5k

spectrogram

How can I isolate these?

Edit: High pass 400, Low pass 700. Noise reduction. Spectral Deletaion.Better. Just need to amplify now. better

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You could try a high pass and low pass filter, and noise reduction. Then amplify the result if needed.

For the high pass filter try a frequency of 400 Hz and for the low pass try 700 Hz. This gives you the segment of 400-700 Hz where your hooting signal seems to be. For noise reduction profile select a part where there is no hooting.

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